Waxaa la ogaaday in α-solanine, oo ah isku-dhis sun ah oo laga helo dhirta baradhada, laga helo α-tomatine-ka dhadhanka qadhaadh leh, kaas oo laga helo dhirta yaanyada, sida lagu sheegay war-saxaafadeed ka soo baxay Jaamacadda Kobe Maarso 26. Cilmi-baarayaal ka socda Jaamacadda Kyoto, Xarunta RIKEN ee Sayniska Kheyraadka Waara iyo Jaamacadda Osaka, ayaa sidoo kale qayb ka ahaa shaqada cilmi-baarista.
Cilmi-baarayaashu waxay baareen waddada biosynthesis-ka α-solanine ee dhirta baradhada. Iyagoo adeegsanaya tafatirka hidde-sideyaasha, waxay carqaladeeyeen hidda-wadaha enzyme-ka biosynthetic-ka ee baradhada si aysan u soo saarin α-solanine. Quudintii α-tomatine ee khalkhal-geliyaha ayaa markaa keentay isbeddel dheef-shiid kiimikaad ah oo u dhexeeya isku-dhafka solanidane ee u dhigma, sida lagu sheegay warbixinta la sii daayay.
Hidde-sidayaasha dioxygenase (DPS) ee ku tiirsan 2-oxoglutarate oo lagu muujiyey baradhada inta lagu jiro isku-darka α-solanine ayaa markaa la soo saaray. Baaritaan dheeraad ah, cilmi-baarayaashu waxay soo saareen dhir wax laga beddelay oo muujinta hidda-wadaha DPS lagu xakameeyay faragelinta RNA.
"Tirada solanidane ee dhirtan baradhada ee wax laga beddelay aad ayay uga hoosaysay kooxda aan wax laga beddelin, spirosolanes-na waxay ku urureen gudaha dhirta halkii ay ka ahaan lahaayeen solanidanes," ayaa lagu yiri war-saxaafadeedka.
Kadib waxaa la cabiray dhaqdhaqaaqa enzymatic-ka ee DPS iyadoo dib loo isku darayo borotiinnada oo lagu muujinayo E. coli. Natiijooyinku waxay muujiyeen doorka gaarka ah ee katalytic-ga ee DPS ee beddelka spirosolane ee solanidane - taasoo caddaynaysa in DPS uu yahay ensaymka ugu muhiimsan ee mas'uulka ka ah beddelkan.
Cilmi-baaristu waxay muujisay sida baradhadu u awoodaan inay soo saaraan α-solanine, sababtoo ah horumarka DPS, kaas oo mas'uul ka ah beddelidda dheef-shiid kiimikaadka spirosolane.
Waxaa la ogyahay in yaanyada ay sidoo kale leeyihiin ensaym lagu dheefshiido spirosolanes. α-tomatine-ka dhadhanka qadhaadh leh waxaa laga helaa yaanyada aan bislaan laakiin waxaa lagu dheefshiidaa esculeoside A oo aan dhadhan lahayn, oo aan sun ahayn marka mirahu bislaadaan, sida lagu sheegay warbixinta.
Baradhada waxaa lagu tilmaamay cunto khatar ah sababtoo ah xaddi badan oo sun ah oo ah α-solanine ayaa sababi kara sumowga cuntada. Natiijooyinka cilmi-baaristan awgeed, waxaa la rajeynayaa inay saldhig u noqon karaan noocyada baradhada mustaqbalka ee lagu xakameynayo bayoolojiga isku-dhafka sunta ah iyadoo la beegsanayo hidda-wadaha DPS.
Rajadu waxay tahay in natiijooyinkan cilmi-baarista loo isticmaali karo taranka baradhada si ay u noqdaan saldhig lagu xakameeyo isku-darka isku-dhafka sunta ah, sida lagu sheegay qoraallada sii deynta.
